How to identify Centella asiatica

Centella asiatica is a powder that is generally light yellow or light brown and can be used to treat injuries or skin problems. Scleroderma can be improved by using Centella asiatica glycoside or applying Centella asiatica glycoside ointment to gradually improve skin problems. However, the amount of Centella asiatica tablets or Centella asiatica powder on the market can vary greatly, which can easily lead to errors and affect usage. Next, let’s take a look at the identification method of Centella asiatica glycoside!

[Assay] Preparation of reference solution: Accurately weigh 20.6 mg of glucose and rhamnose dried to constant weight.

⒐4mg, put in a 100ml volumetric flask, add water to dissolve and dilute to the scale, shake well, and you are done.

Preparation of standard curve: Accurately measure 1.0, 2.0, 3.0, 4.0, 5.0 ml of reference solution and place them in 50 ml

In the stoppered test tube, add water to 5 ml, accurately add 5 ml of copper solution and shake well, heat in a boiling water bath for 15 minutes, take out,

Cool, add 5 ml of sulfuric acid solution (0.5 mol/L), place in an ice bath and shake thoroughly to dissolve the cuprous oxide precipitate completely.

Titrate with sodium thiosulfate solution (0.005 mol/L). When it is close to the end point, add 1 to 2 drops of starch indicator solution and continue titrating until the blue

The color disappears, take another 5ml of water and operate in the same way as a blank, and use the difference in ml of the two titrations as the ordinate.

The standard curve was drawn with the number as the horizontal axis.

Determination method Take 0.1g of the product, weigh accurately, add 10ml of 10% dilute sulfuric acid solution to dissolve, and place on a double-layer asbestos board.

Heat to reflux over direct fire, boil for 1 hour, cool, neutralize with 20% sodium hydroxide solution, transfer to a 250ml volumetric flask, add

Dilute with water to the mark, shake well, filter, accurately measure 5 ml of the filtrate and place it in a 50 ml stoppered test tube, and prepare the standard curve according to the following steps:

Follow the same procedure starting from “precisely add 5 ml of copper test solution”. Take another 0.1g of the product and place it in a 250ml volumetric flask.

Dilute with water to the scale and proceed in the same way. Take the difference in ml between the two titrations and read the equivalent mg ​​of sugar from the standard curve.

Divide by 0.5468 to get the total glycosides in mg, and calculate to get.

Calculated on the dry basis, the total glycosides, calculated as Centella asiatica, shall not be less than 60.0%.

The above is the identification method of Centella asiatica glycoside. If you have just had surgery, you can also use Centella asiatica glycoside to promote wound healing. But if you don’t know how to use Centella asiatica powder, it’s best not to use it casually. Some burns and difficult-to-remove scars can also be gradually improved by using Centella asiatica powder, which can have a relatively good effect in lightening scars.