Meropenem instructions

Meropenem is a relatively common drug in normal times. The ingredients of this drug are composed of chemical elements and rare elements. It can achieve the effect of antibiotics because it can kill bacteria and resist toxins. It can also promote the recovery of qi and blood in the body. However, everyone needs to pay attention to use this drug for symptomatic purposes because this drug is rarely seen in life.

Packaging; Moulded antibiotic glass bottles.

Storage: Store tightly closed in a cool, dark and dry place.

Pharmacological action: Meropenem is a synthetic broad-spectrum carbapenem antibiotic that produces antibacterial effects by inhibiting the synthesis of bacterial cell walls. Meropenem can easily penetrate the cell walls of most Gram-positive and Gram-negative bacteria to reach its target penicillin-binding protein (PBPS). In addition to metallo-β-lactamase, it has strong stability to the hydrolysis of most β-lactamases (including penicillinase and cephalosporinase produced by Gram-positive and Gram-negative bacteria). Meropenem should not be used to treat methicillin-resistant Staphylococcus aureus infections, and sometimes shows cross-resistance to other carbapenem-resistant strains. In vitro experiments have shown that meropenem combined with aminoglycoside antibiotics can produce a synergistic effect against some isolated strains of Pseudomonas aeruginosa.

Determination method Method name: Determination of meropenem-high performance liquid chromatography

Scope of application: This method uses HPLC to determine the content of meropenem (C17H25N3O5S·3H2O).

This method is applicable to meropenem.

Principle of the method: The test sample is made into a mobile phase solution and enters into a high performance liquid chromatograph for chromatographic separation. The absorption value of meropenem is detected at a wavelength of 220nm using an ultraviolet absorption detector to calculate its content.

Reagents: 1. Acetonitrile

2. 0.3% triethylamine solution

Instruments and equipment: 1. Instruments

1.1 High Performance Liquid Chromatography

1.2 Chromatographic columns

Octylsilane bonded silica gel is used as filler, and the separation degree between meropenem peak and meropenem ring-opening product peak should meet the requirements.

1.3 UV absorption detector

2. Chromatographic conditions

2.1 Mobile phase: 0.3% triethylamine solution in acetonitrile = 100 7,

2.2 Detection wavelength: 220nm

2.3 Column temperature: room temperature

Sample preparation:

1. 0.3% triethylamine solution

Take 3.0 mL of triethylamine, dilute to 900 mL with water, adjust the pH to 5.0 with phosphoric acid solution (1→10), and dilute to 1000 mL with water.

2. Weigh the test sample

Accurately weigh an appropriate amount of this product.

3. Preparation of reference solution

Accurately weigh an appropriate amount of meropenem, add mobile phase to dissolve and make a solution containing 0.5 mg per 1 mL, shake well, and the solution is ready.

4. Preparation of test solution

Dissolve the sample in the mobile phase to make a solution containing 0.5 mg per 1 mL, shake well, and you have it.

Note: "Precision weighing" means that the weight should be weighed accurately to one thousandth of the weight taken. "Precision measurement" means that the accuracy of the measured volume should meet the accuracy requirements of the pipette for that volume in the national standard.

Operation steps: Accurately pipette 10 μL of the reference solution and the test solution respectively and inject them into the high performance liquid chromatograph. Use the ultraviolet absorption detector to measure the absorption value of meropenem at a wavelength of 220 nm and calculate its content.