Anti-double-stranded DNA antibody negative

Anti-double-stranded DNA antibody is a type of anti-DNA antibody. This antibody is of relatively important significance in clinical examinations. It can perform good examinations of kidney damage and is a sign of kidney damage examination. In addition, it is also of certain value in judging the prognosis. For example, patients with systemic lupus erythematosus, chronic active hepatitis, connective tissue diseases, etc., all undergo this examination.

Abnormal results of clinical significance : Anti-dsDNA autoantibodies are the most important autoantibodies in SLE, with a detection rate of 40%-70%. The presence of high titer anti-dsDNA is an important basis for the diagnosis of SLE and is also a sign of disease activity, especially kidney damage. In addition to being used for the diagnosis of SLE, it can also be used to monitor the clinical course and treatment effects, and has certain value in determining prognosis. However, some people have reported finding low-titer anti-dsDNA positivity or increased levels in patients with liver disease, juvenile rheumatoid arthritis, and even in normal people: this may be systemic lupus erythematosus, and can also be seen in other connective tissue diseases, chronic active hepatitis, etc. People who need to be checked: those who suffer from the above diseases

Precautions and requirements during inspection: It is generally believed that the titer of anti-double-stranded DNA is parallel to the disease state, that is, when the disease is active, the titer of anti-DNA antibodies increases, and when the disease is relieved, the titer decreases. Because the measurement methods vary from place to place, the normal values are also different. Generally speaking, the binding rate must be greater than 20% to be clinically significant. The specificity of any detection technique is not 100%. For example, although the matrix of the green fly short membrane does not contain ssDNA, it may contain a small amount of histones. The dsDNA used as an antigen in radioimmunoassay, ELISA, gold filtration, etc. often contains ssDNA. Even if the contaminating ssDNA is less than 1%, the false positive rate caused can be as high as 6%. Therefore, the results of anti-dsDNA testing should be combined with clinical analysis and, if necessary, dynamic observation should be performed. In addition, given the different sources of DNA antigens in the detection reagents, the base composition and sequence may vary to a certain extent, causing changes in the epitope to which the antibody binds.

Inspection process and inspection methods: There are many methods for determining anti-double-stranded dSDNA, such as immunodiffusion, countercurrent immunoelectrophoresis, agglutination test, biotin binding test, indirect immunofluorescence, radioimmunoassay, enzyme-linked immunosorbent assay, etc. The most commonly used ones are radioimmunoassay, indirect immunofluorescence and enzyme-linked immunosorbent assay.

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