BSA

Blood contains many components. The lack of any component will cause serious damage to human health and may also cause blood diseases. Therefore, in daily life, diet must be correct, which will be of great help to all aspects of the body and enable the body to develop healthily. What is bovine serum albumin? Many people don't know much about it, and problems need to be improved in a timely manner.

Bovine serum albumin is also very helpful to the human body, so when choosing it, you need to have a comprehensive understanding of it, so that when supplementing the human body with such substances, you will know how to do the best.

Bovine serum albumin:

A simple protein in bovine serum, it is the main component of blood (38g/100ml) and has a molecular weight of 68kD. Isoelectric point 4.8. The nitrogen content is 16% and the sugar content is 0.08%. It only contains hexose and glucosamine, and the fat content is only 0.2%. Albumin is composed of 581 amino acid residues, of which 35 cysteines form 17 disulfide bonds, and there is a free thiol group at the 34th position of the peptide chain. Albumin can bind to a variety of cations, anions and other small molecules.

Albumin in the blood mainly plays the role of maintaining osmotic pressure, pH buffering, carrier and nutrition. In serum-free culture of animal cells, the addition of albumin can play a physiological and mechanical protective role and a carrier role. Bovine serum albumin (BSA), also known as the fifth fraction, is a globulin in bovine serum, containing 583 amino acid residues, with a molecular weight of 66.430 kDa and an isoelectric point of 4.7. Bovine serum albumin is widely used in biochemical experiments, such as as a blocking agent in western blot.

Production method:

Serum is separated from bovine blood, precipitated with ammonium sulfate, and then purified by caprylic acid treatment.

Through the above introduction, we have a good understanding of what bovine serum albumin is. What is its production method? The above also has a detailed explanation. However, such things cannot be used casually. You must choose according to your own needs. This will be of great help to your own health.