Ovulation test strip principle

In today's world of particularly high competitive pressure, I believe that many young couples who have just gotten married and are just starting their careers do not want to have children too early. Therefore, couples always take a lot of safety measures during sexual intercourse, because they don’t want the baby to get pregnant accidentally. At this time, scientists invented ovulation test strips, which can be used to measure the time of contraception.

Rationale

This reagent adopts the principle of immunochromatography double antibody sandwich method and colloidal gold labeling technology. It is made of nitrocellulose membrane with mouse anti-a-LH monoclonal antibody (test line) and goat anti-mouse IgG polyclonal antibody (control line) as solid phase, glass fiber pre-adsorbed with colloidal gold labeled mouse anti-β-LH monoclonal antibody and other auxiliary materials pasted in sequence.

During the test, the LH in the urine sample combines with the pre-solidified β-LH monoclonal antibody colloidal gold on the sample pad of the test paper to form a complex. The complex migrates upward to the test line (T) area due to the capillary action of the membrane and reacts with the a-LH antibody on the membrane to form a red strip. Regardless of whether LH is present in the sample, when the liquid level continues to migrate to the control line (C) area, a red strip will definitely appear in this area. If the color of the red strip in the test line area (T) is darker or similar to that of the red strip in the control line area (C), it indicates a positive result, indicating that the subject is in the ovulation period; if the color of the red strip in the test line area (T) is lighter than that of the red strip in the control line area (C), it indicates a negative result, indicating that the subject is not in the ovulation period. The red stripe in the control line area (C) is the standard for judging whether there is enough specimen and whether the chromatography process is normal. It also serves as the internal control standard of the reagent.

When to use

Ovulation test strips mainly measure the hormone luteinizing hormone (LH) in women's bodies, detecting the peak level of luteinizing hormone so that women can predict the best time for conception or contraception.

It is recommended to start testing on the tenth day of menstruation and test once a day with an ovulation test strip. If you find that the test is gradually getting stronger, you should increase the frequency of testing. It is best to test every 4 hours. Try to measure a strong positive and seize the moment when the strong positive turns weak. Ovulation occurs when the strong positive turns weak. If you find that it is weakening rapidly, it means that the egg is about to break out of the shell, so you have to hurry! Ovulation will definitely occur within 24 hours, and you cannot drink water 2 hours before taking the ovulation test. Do not test with morning urine, it is best to test between 10-20 o'clock.

After the strong positive turns weak, continue to test the ovulation test paper. Don't be afraid of wasting the ovulation test paper. Keep testing until it becomes negative. You can rest for a few days at this time. Start testing the ovulation test paper every day 10 days after ovulation. If a strong positive is found, the hope of pregnancy is great. You can use an early pregnancy test paper. Generally, the early pregnancy test paper at this time will be a weak positive. If you need to do B-ultrasound monitoring, it is best to go to the hospital for B-ultrasound immediately if the ovulation test paper shows a positive result.

I used to think that ovulation test strips were only used to measure the duration of contraception. After reading this article, I found that it has more than just this function, it can also predict the best time for conception. I didn't expect that a small ovulation test paper could be so effective. Whether you want to have sex or want to know the best time to conceive, you can try the ovulation test strips.